BBa_K1621005

BBa_K1621005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1621005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ramona Emig, Rabea Jesser, Julika Neumann, Lara Stuehn
Date created: 2015-08-28 11:00:00
Date modified: 2015-08-31 01:30:30

TpF1 - bacterioferritin derived from Treponema pallidum



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1621005_sequence (Version 1)

Description

This part contains the coding sequence of a bacterioferritin monomer derived from Treponema pallidum, TpF1. It was isolated from the subspecies pallidum which causes syphilis, a disease that is transmittable by sexual contact or congenitally. Worldwide, more than 12 million new cases of syphilis are reported which underlines the need of high detection methods that are highly sensitive and specific as well as easy to perform.

TpF1 was shown to be highly immunogenic in humans and rabbits (McGill et al., 2010), what was the reason for Jiang et al. (2013) to use it for the establishment of a diagnostic ELISA for clinical applications.

Bacterioferritins in general are central players in bacterial iron metabolism. Their functions reach from detoxification of iron in the cell to iron storage and even protection against oxidative stress (Carrondo, 2003). Disuflide bonds and hydrophobic interactions mediate the formation of homo-24-mers of about 190 kDa in the outer membrane (Radolf et al. 1987). Treatment with β-mercaptoethanol or boiling in SDS leads to dissaggregation of the complex, resulting in smaller oligomers (~160 kDa) and monomers (~19 kDa). This part???s sequence encodes for such a monomer (Jiang et al. 2013).

The sequence was synthesized by Integrated DNA Technologies in a variant that was codon optimized for expression in Escherichia coli with the respective tool from IDT. It was shipped to the registry in standard pSB1C3 and begins with a start codon (ATG). Cloning into the shipping backbone was performed by Gibson Assembly.

Notes

Before synthesis the sequence was codon optimized for expression in E. coli with the codon optimization tool from IDT.

Source

The part's sequence was obtained from Jiang et al. (2013) and synthesized by Integrated DNA Technologies.

Sequence Annotation Location Component / Role(s)
TpF1
4,531
feature/cds CDS
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1621005/1