BBa_K1621006

BBa_K1621006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1621006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ramona Emig, Rabea Jesser, Julika Neumann, Lara Stuehn

Date created: 2015-08-27 11:00:00

Date modified: 2015-09-11 05:56:16

Dihydroxyacid dehydratase (Salmonella Typhimurium)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1621006_sequence (Version 1)

Description

This part contains the coding sequence of the enzyme dihydroxyacid dehydratase from //Salmonella typhimurium//. Its main biological function is the dehydratation of 2,3-dihydroxy-isovaleic acid into alpha-ketoisovaleric acid in the biosynthesis of isoleucine and valine.
Additionally, the protein is immunogenic in humans as it was shown by the group of Prof. Dr. Michael Hust at TU Braunschweig (Meyer //et al.//, 2012). This is why the part can be used for diagnostic purposes concerning //S. typhimurium//. The sequence as well as an expression plasmid carrying its sequence in a His-tagged variant was obtained directly from this group.

Notes

Two recognition sites for PstI have been removed by site-directed mutagenesis. Therefore, the guanine residues at positions 96 and 679 have been mutated into cytosines. This results in one silent mutation and an amino acid exchange from alanine to proline at position 227 of the primary structure.

Source

The part is derived from Salmonella typhimurium. The sequence as well an expression plasmid carrying the part in a His-tagged variant were obtained from Meyer et al. (2012).

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1621006/1

Member of these Collections

• iGEM Parts Registry