Types | DnaRegion
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Roles | engineered_region
Device
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Sequences | BBa_K1627002_sequence (Version 1)
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Description
This part contains genes that allow for the demethylation of 7-methylxanthine. The part contains a synthetic operon consisting of ndmC, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.
This part can be used in a ∆guaB strain of E. coli to measure the amount of 7-methylxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of 7-methylxanthine, a growth curve can be made that correlates culture growth with concentration of 7-methylxanthine. Then if the strain is grown in a minimal media with an unknown concentration of 7-methylxanthine, the growth can be compared to the growth curve to calculate the initial concentration of 7-methylxanthine in the media.
Notes
These genes were amplified from the pDCAF3 plasmid (BBa_K734000). The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.
Source
The ndmC and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.