| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K163000_sequence (Version 1)
|
Description
Gene t encodes a holin, which is considered to degrade or alter the cytoplasmic membrane, thus allowing lysozymes from T4 gene e to reach the periplasm and gain access to the peptidoglycan layer. Essential component of the cell-lysis system triggered by T4 bacteriophage in E. coli. Expression induces cell-lysis.
Notes
A protein that could effectively trigger cell-lysis in E. coli upon selective induction. When looking for a cell-lysis pathway, system had to be shown to work in E. coli, be non-lethal until induction, and act quickly once triggered. This particular gene was designed by Morita, et al to turn on following inducible expression of a target protein, a feature that could be used to easily purify a protein of interest. Furthermore, Morita, et al report that cell-lysis proceeded efficiently at both mid or late logarithmic growth phase.
Source
Sequence from paper "Programmed Escherichia coli Cell Lysis by Expression of Cloned T4 Phage Lysis Genes " by Morita, et al. Biotechnol. Prog., 17 (3), 573 -576, 2001. Gene bank accession number Y00408.
