| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K163002_sequence (Version 1)
|
Description
This is the protein Mefp-5 that is secreted by Mytilus Edulis. Mefp-5 is one of the strongest adhesives naturally created, and a powerful anti-biofouling agent. The DNA has been codon optimized for use in E. coli using open-software codon-optimizer at http://genomes.urv.es/OPTIMIZER/. This gene lacks a promoter sequence.
Notes
This sequence has been codon-optimized for production in E. coli. Mefp-5 contains over 20% molar concentration of L-DOPA, the highest DOPA content of known adhesive proteins secreted by Mytilus Edulis. Mefp-5 also has strong anti-biofouling properties, that could possibly be utilized by such things like pacemakers or heart monitors. Mefp-5 is difficult to obtain from purification of mussel proteins, so being able to obtain Mefp-5 from genetic engineering methods will allow researchers to better learn its properties.
Source
Waite, et al. "Polyphosphoprotein from the adhesive pads of Mytilus edulis" Biochemistry 40 (9), 2887-2893 (2001). Nucleotide sequence from GeneBank, accession number AF333078
