BBa_K163101

BBa_K163101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K163101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel M. Choi

Date created: 2008-10-10 11:00:00

Date modified: 2015-05-08 01:10:55

pUC8 Plasmid DNA

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_K163101_sequence (Version 1)

Description

pUC8 is a plasmid that has the last 336 AA of RsaA (Caulobacter surface protein) and it allows for a cloning of desired segment into a EcoR1, Sma1 and or BamH1 site upstream of the 336.

Notes

This plasmid is used for the Caulobacter secretion system. We use this as the main template in which we splice in our BioBricks for Caulobacter.

Source

Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived
system for insertion mutagenesis and sequencing with synthetic universal
primers. Gene 19:259???268.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
EcoRI	2016,2021	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K163101/1

Member of these Collections

• iGEM Parts Registry