| Types | DnaRegion
|
| Roles | RNA
mature_transcript_region
|
| Sequences | BBa_K1633007_sequence (Version 1)
|
Description
This part is a short hairpin RNA (shRNA) sequence. When this shRNA sequence is cut by restriction enzyme and then integrated into pcDNA 6.2 vector, this shRNA can play a RNAi function in mammalian cell lines such as HEK293 cell. When the shRNA vector of MOR is transfected into HEK293 cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at GFP mRNA under the guide of siRNA sequence and cleave the GFP mRNA.
Notes
We designed specific GFP siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene GFP to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid.
Source
We designed the GFP 122 siRNA and ordered the part from a DNA synthesis company.
