BBa_K1633008

BBa_K1633008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1633008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei

Date created: 2015-09-12 11:00:00

Date modified: 2015-09-16 05:46:23

coding sequence to express nSMase 2 protein.

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1633008_sequence (Version 1)

Description

This part is a codon-optimized nSMase 2 gene CDS. When this part is inserted into pcDNA 3.1 vector and transfected into mammalian cells,it can express nSMase2 gene in mammalian cells and stimulate the secretion of exosomal siRNAs from cells.

Notes

There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements.

Source

Homo Sapiens. The original sequence is from NCBI. NCBI Gene ID is 55512. The sequence codon of this part was optimized. We ordered the sequence from a DNA synthesis company

Other Properties

• igem#experience: Works
• igem#status: Planning
• rdf-schema#seeAlso: Q9NY59
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1633008/1

Member of these Collections

• iGEM Parts Registry