| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K1634002_sequence (Version 1)
|
Description
Pmyo2 is a promoter which can drive the expression in the muscle of C.elegans especially the muscle in the head and pharynx.And we link the Pmyo2 with some channelrhodopsin which can be activated or supressed by the light. Since we have learned that the chosing of direction in C.elegans depends on the muscle in the head, we can observe the obvious change in moving pattern of the C.elegans after we shed the light. In other words,we can use them to construct a light-sensed locomotion controlling system in C.elegans. And we achieve that goal by using this part to control the movement of the head, the movement of the whole C.elegans.
Notes
Since we use the restriction enzyme site to do the ligation, we should pay attention to the sequence of Pmyo2, avoiding using the restriction site exits in it.
Source
We get Pmyo2 from the C.elegans' genomic DNA by PCR.
