| Types | DnaRegion
|
| Roles | DNA
sequence_feature
|
| Sequences | BBa_K1636002_sequence (Version 1)
|
Description
This linker was designed by the iGEM TecCEM team in order to retrieve single-stranded oligonucleotides when produced as single-stranded genetic material (e.g. plasmid). The linker should be flanking said oligonucleotides, and given the design of its sequence, it is able to form a hairpin-like secondary structure that allows the usage of a restriction enzyme (MlyI from New England BIolabs) with a blunt cut thereby releasing the oligos of interest.
Notes
The design considerations of this linker included the ability of forming a stable hairpin-like secondary structure that provided with the enzyme restriction site of MlyI which could make a blunt cut without leaving scar on the oligonucleotides of interest.
Source
This part was designed by the iGEM TecCEM team from scratch and is not based or does not come from any genome.
