BBa_K1638003

BBa_K1638003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1638003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jens Sivk??r Pettersen

Date created: 2015-05-08 11:00:00

Date modified: 2015-09-18 10:36:33

T25 domain of cyaA from Bordetella pertussis (IPTG inducible)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1638003_sequence (Version 1)

Description

The T25 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by IPTG.
When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the protein associates, T25 and T18 associates too, and convert ATP to cAMP.

Notes

asd

Source

asd

Components

Access	Instance	Definition
public	BBa_K1638000	pLac
public	BBa_K1638001	BBa_K1638001
public	BBa_K1638002	T25 domain

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1638000	1,142	pLac
BBa_K1638001	143,161	BBa_K1638001
BBa_K1638002	162,848	T25 domain

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1638003/1

Member of these Collections

• iGEM Parts Registry