BBa_K1639006

BBa_K1639006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1639006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Yılmaz
Date created: 2015-09-11 11:00:00
Date modified: 2015-09-21 05:08:59

PotB59-pomA



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1639006_sequence (Version 1)

Description

PotB59, chimeric protein that is formed by the N-Terminal region of PomB and the C-terminal of E. coli MotB

It was shown that a chimeric protein, PotB, renamed as PotB59, that has the N-terminal region of PomB (1???50) fused to the C-terminal periplasmic region of E. coli MotB (59???308), is functional with PomA in E. coli as well as in strains of V. alginolyticus that are defective in motX or motY (Asai et al. 2003) Instead of the hydrogen-dependant MotA MotB Stator protein partially adapted from the V. alginolyticus, sodium-dependant Stator proteins were installed. After this adaptation, the flagellar velocity increased by twice fold.

Notes

This part contains an additional BamHI restriction site at beginning of TlpB sequence and a XhoI restriction site at the end.

Source

asd

Sequence Annotation Location Component / Role(s)
PotB59
PomA
Ribosome Reinitiation Spacer
RBS
9,911
965,1723
915,935
936,958
CDS feature/cds
CDS feature/cds
ribosome_entry_site feature/rbs
ribosome_entry_site feature/rbs
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1639006/1