Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nurgeldi Bazarov
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 12:20:03
mammalian LacI with miR-373 binding site
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1639010_sequence (Version 1) |
Description
In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-373 rate. In gastric epithelial cells level of this mirna is low but in gastric cancer cells level is very high.Notes
We inserted two Mlu1 cut sites on each side of miR-373 binding so that other iGEM teams could easily replace it with any sequence.Source
This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators.| Sequence Annotation | Location | Component / Role(s) |
| Kozak mammalian LacI SV40 NLS Stop Codon miR-373 binding site ATG | 1,6 7,1125 1102,1122 1123,1134 1141,1163 7,9 | feature/rbs ribosome_entry_site CDS feature/protein feature/tag tag feature/stop stop_codon non_covalent_binding_site feature/binding start_codon feature/start |