Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nurgeldi Bazarov
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-18 06:55:31
T7 expression system for pSB1C3
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1639012_sequence (Version 1) |
Description
In our project we needed to express two different genes at the same bacteria, but unfortunately because of high repeats of sequences gene synthesizing company couldn't synthesize it. To solve this we just divided our genes into different plasmids and cotransformed it into same bacteria. For coexpression of genes from different plasmids origins have to be compatible, like ColA and ColE1.pSB1C3 possess ColE1 origin of replication. So it's compatible for expression with plasmid containg ColA origin of replication. But it doesn't contain expression machinery like promoters, terminators. We inserted this part to modify pSB1C3 so that it can be used as expression vector.
Notes
We inserted BamH1 and Sal1 cut sites between T7 promoter and T7 terminator. This part also contain RBS. Any other team can use this part to modify vectors like pSB1C3 to add T7 expression feature. Just clone it with enzymes at prefix and suffix.Source
It is combination of T7 promoter-terminator with sequences containing restriction enzyme cut sites.| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter T7 bacteriophage RBS T7 terminator | 1,19 35,57 124,171 | feature/promoter promoter feature/rbs ribosome_entry_site feature/stem_loop stem_loop |