Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nurgeldi Bazarov
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-21 04:46:20
GFP with an ASV degradation tag with a half-life of ~110 min
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1639023_sequence (Version 1) |
Description
GFP is seemed to be very stable once produced. But ASV tagged GFP is susceptible to action of intracellular proteases which reduce it's half-life approximately to 110 min. It's useful for studying of real-time gene expression in individual cells.Notes
We alreay designed it in tagged form. Anybody can express it with given sequence directly.Source
This part is ASV tagged version of jellyfish Aequorea victoria.| Sequence Annotation | Location | Component / Role(s) |
| GFP ASV tag ATG Stop Codon T7 terminator | 1,714 715,753 1,3 754,756 793,840 | feature/protein CDS feature/tag tag start_codon feature/start stop_codon feature/stop feature/stem_loop stem_loop |