BBa_K1639024

BBa_K1639024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1639024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nurgeldi Bazarov
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-21 04:46:08

Toehold riboregulator without reporter



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K1639024_sequence (Version 1)

Description

This part is composed of approximately 100 nt RNA molecule. It's 30 nt is completely complementary to BBa_K1639001. Together they constitute AND logic gate part of our project. It's a new and flexible approach to AND gate switches because of sequence variablity and ease of modification.

Notes

30 nt (87-116bp) in 3' end of molecule is very critical to function properly. We noticed it in features.

Source

Labaroty engineered Toehold riboregulator is syntheticaly produced RNA molecule which designed complementary to trigger RNA (BBa_K1639001)

Sequence Annotation Location Component / Role(s)
T7
Toehold-RNA
RBS
Conserved region
21 nt linker
GFP-9nt
ATG
1,19
20,107
61,68
87,116
87,107
108,116
75,77
feature/promoter promoter
stem_loop feature/stem_loop
feature/rbs ribosome_entry_site
feature/conserved conserved_region
sequence_feature feature/misc
feature/protein CDS
start_codon feature/start
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1639024/1