BBa_K1639024

BBa_K1639024 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1639024

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nurgeldi Bazarov

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-21 04:46:08

Toehold riboregulator without reporter

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_K1639024_sequence (Version 1)

Description

This part is composed of approximately 100 nt RNA molecule. It's 30 nt is completely complementary to BBa_K1639001. Together they constitute AND logic gate part of our project. It's a new and flexible approach to AND gate switches because of sequence variablity and ease of modification.

Notes

30 nt (87-116bp) in 3' end of molecule is very critical to function properly. We noticed it in features.

Source

Labaroty engineered Toehold riboregulator is syntheticaly produced RNA molecule which designed complementary to trigger RNA (BBa_K1639001)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7	1,19	feature/promoter, promoter
Toehold-RNA	20,107	stem_loop, feature/stem_loop
RBS	61,68	feature/rbs, ribosome_entry_site
Conserved region	87,116	feature/conserved, conserved_region
21 nt linker	87,107	sequence_feature, feature/misc
GFP-9nt	108,116	feature/protein, CDS
ATG	75,77	start_codon, feature/start

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1639024/1

Member of these Collections

• iGEM Parts Registry