BBa_K1641004

BBa_K1641004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-08 11:00:00

Date modified: 2015-09-09 12:14:36

Fusion protein of Cre::EGFP::ssra-tag, with RBS at beginning

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K1641004_sequence (Version 1)

Description

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Cre::EGFP::ssra-tag.

Cre is a most commonly used recombinase with invertase activity that turns the sequence between two anti-parallel LoxP sites up-side-down. A EGFP fusion at the C-term of Cre has been proved by us not to significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need.

This part is for expression of Cre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.

Notes

Ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. C-term EGFP fusion on Cre work pretty good for activity, which we proved.

Source

Cre from BBa_K1179058 and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
conserved	5,8	conserved_region, feature/conserved
BBa_B0034	1,12	feature/BioBrick, engineered_region

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641004/1

Member of these Collections

• iGEM Parts Registry