Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pai Li
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-13 10:53:02
Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1641006_sequence (Version 1) |
Description
This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Vcre::EGFP::ssra-tag. The part is for expression of Vcre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.Vcre (BBa_1641000) in our bricks is a new-discovered Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel VloxP sites up-side-down. A EGFP fusion at the C-term of Vcre will not significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression.
Notes
Ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Vcre is according to its structure is similar to Cre, and hence the C-term EGFP fusion tend not to interfere the activity.Source
Vcre from BBa_1641000 (de novo synthesized gene) and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks.| Sequence Annotation | Location | Component / Role(s) |
| B0034 Vcre Flexible chain EGFP::ssra | 1,12 19,1158 1159,1182 1183,1941 | feature/rbs ribosome_entry_site CDS feature/protein feature/misc sequence_feature feature/protein CDS |