BBa_K1641007

BBa_K1641007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-08 11:00:00

Date modified: 2015-09-13 10:52:29

Fusion protein of Scre::EGFP::ssra-tag, with RBS at beginning

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1641007_sequence (Version 1)

Description

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Scre::EGFP::ssra-tag. The part is for expression of Scre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.

Scre (BBa_K1641001) in our bricks is a new-discovered Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel SloxP (or its derivatives) sites up-side-down. An EGFP fusion at the C-term of Scre will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. An ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression.

Notes

Flexible chain works to keep two parts away to avoid misfolding; ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Scre has similar structure of Cre, and hence have far better performance if EGFP is fused to its C-term rather than N-term.

Source

Scre from BBa_K1641001 and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks.

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641007/1

Member of these Collections

• iGEM Parts Registry