BBa_K1641010

BBa_K1641010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 12:43:19

Rox, recognition site of recombinase Dre

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K1641010_sequence (Version 1)

Description

This is the recognition site of Cre-like recombinase Dre, that is "TAACTTTAAATAATTGGCATTATTTAAAGTTA". This recognition site is not homologous with LoxP and hence will not cross-react with Cre or other common recombinases.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.

Notes

Vox do not process start coding ATG, so the original Rox sequence just works fine.

Source

By de novo synthesis.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641010/1

Member of these Collections

• iGEM Parts Registry