BBa_K1641014

BBa_K1641014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 07:33:10

Up-side-down RBS::mcherry-ssra with loxP recognition sites.

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K1641014_sequence (Version 1)

Description

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two LoxP sites at the upstream and downstream of the gene. LoxP can be recognized and the middle sequence can be restored by invertase Cre. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Notes

This sequence is up-side-down, hence can be inverted into normal sequence.

Source

RBS and loxP are added to mcherry by cut-and-link or PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LoxP	1,34	non_covalent_binding_site, feature/binding
Inverted mcherry-ssra	44,787	CDS, feature/cds
BBa_B0034	794,805	feature/rbs, ribosome_entry_site
LoxP	812,845	non_covalent_binding_site, feature/binding

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641014/1

Member of these Collections

• iGEM Parts Registry