BBa_K1641016

BBa_K1641016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 11:17:59

Up-side-down RBS::mcherry-ssra with Rox, recognition site of Dre

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1641016_sequence (Version 1)

Description

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two Rox sites at the upstream and downstream of the gene. Rox can be recognized and the middle sequence can be restored by invertase Dre (BBa_K1641002). Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Notes

This sequence is up-side-down, hence can be inverted into normal sequence.

Source

Constructed by PCR or cut-and-link of previous bricks.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Rox	1,32	non_covalent_binding_site, feature/binding
inverted mcherry-ssra	42,785	feature/cds, CDS
B0034	792,803	feature/rbs, ribosome_entry_site
Rox	810,841	feature/binding, non_covalent_binding_site

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641016/1

Member of these Collections

• iGEM Parts Registry