BBa_K1641017

BBa_K1641017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-10 11:00:00

Date modified: 2015-09-11 09:50:19

Up-side-down RBS::mcherry-ssra with Vox, Vika recognition sites

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region

Description

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two Vox sites at the upstream and downstream of the gene. Vox can be recognized and the middle sequence can be restored by invertase Vika (BBa_K1641003). Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Notes

This sequence is up-side-down, hence can be inverted into normal sequence.

Source

Constructed by PCR or cut-and-link of previous bricks.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641017/1

Member of these Collections

• iGEM Parts Registry