BBa_K1641018

BBa_K1641018 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 11:58:35

Up-side-down RBS::mcherry-ssra with SloxM1, Scre recognition sites

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1641018_sequence (Version 1)

Description

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two SloxM1 sites at the upstream and downstream of the gene. SloxM1 can be recognized by invertase Scre and the middle mcherry can be restored through inversion. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Notes

This sequence is up-side-down, hence can be inverted into normal sequence.

Source

This part is constructed by PCR or cut-and-link of biobricks.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SloxM1	1,34	non_covalent_binding_site, feature/binding
inverted mcherry-ssra	44,787	feature/cds, CDS
B0034	794,805	ribosome_entry_site, feature/rbs
SloxM1	812,845	feature/binding, non_covalent_binding_site

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641018/1

Member of these Collections

• iGEM Parts Registry