BBa_K1641220

BBa_K1641220 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1641220
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianheng Liu
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 08:13:25

pBAD(forward)-FRT(forward)-loxP(reverse)



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K1641220_sequence (Version 1)

Description

This sequence has two primer sites and a restriction enzyme site between FRT and loxP for further testing in our experiment.
Two primers are:
GCACCTTGACTCTGACAATCCT (forward)
GCCTGTGCTAATGGTGATGACT (reverse)
they both have a high annealing temperature (about 58C). They can be used as qPCR primers.
The restriction enzyme sites are HindIII and BanI.

Source

Synthesized

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1641220/1