Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianheng Liu
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 08:13:25
pBAD(forward)-FRT(forward)-loxP(reverse)
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1641220_sequence (Version 1) |
Description
This sequence has two primer sites and a restriction enzyme site between FRT and loxP for further testing in our experiment.Two primers are:
GCACCTTGACTCTGACAATCCT (forward)
GCCTGTGCTAATGGTGATGACT (reverse)
they both have a high annealing temperature (about 58C). They can be used as qPCR primers.
The restriction enzyme sites are HindIII and BanI.