BBa_K1641999

BBa_K1641999 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641999

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chunyang Ni

Date created: 2015-09-10 11:00:00

Date modified: 2015-09-18 12:55:23

pAUR135 shuttle vector RFC10 optimized

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K1641999_sequence (Version 1)

Description

pAUR135 RFC25 is a modified shuttle vector including DNA sequence to remove the vector region containing selectable marker AUR1-C from transformants of S.cerevisiae by this vector which allows repeatedly transformation. With optimization, K1641999 can be applied on RFC standard.

Notes

mutation and MCS modification

Source

DNA synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
AurR	2822,4027	feature/cds, CDS
GAL10 promoter	4434,5098	sequence_feature, feature/misc
Lac operator	2424,2478	operator, feature/operator
AmpR	481,1342	CDS, feature/cds
ori	1513,2100	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641999/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors