BBa_K1648001

BBa_K1648001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1648001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Leung King Pong

Date created: 2015-07-13 11:00:00

Date modified: 2015-09-15 07:51:31

Recombination Template for mamXY, mamGC and mms Operons

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K1648001_sequence (Version 1)

Description

This part is constructed by six fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence of mamXY, mamGC and mms operon(a part of genome from magnetospirillum gryphiswaldense magnetosome island).

Notes

In order to form a homologous recombination template, we select 250 bp of each fragment as the flanking sequence and do the overlapping PCR to ligate fragments together.

Source

Magnetospirillum gryphiswaldense MSR-1 v2, complete genome

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mamY 5' flanking sequence	1,250	feature/misc, sequence_feature
ftsZ-like 3' flanking sequence	251,500	feature/misc, sequence_feature
mamG 5' flanking sequence	501,750	sequence_feature, feature/misc
mamC 3' flanking sequence	751,1000	sequence_feature, feature/misc
mms6 5' flanking sequence	1001,1300	feature/misc, sequence_feature
mms48 3' flanking sequence	1301,1550	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1648001/1

Member of these Collections

• iGEM Parts Registry