BBa_K165030

BBa_K165030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K165030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aaron Glieberman

Date created: 2008-10-28 12:00:00

Date modified: 2015-05-08 01:10:56

mCYC promoter plus Zif268-HIV binding sites

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K165030_sequence (Version 1)

Description

This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.

Notes

It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.

Source

Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Zif268-HIV binding sites (3)	1,46	feature/binding, non_covalent_binding_site

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K165030/1

Member of these Collections

• //chassis/eukaryote/yeast
• //direction/forward
• //promoter
• //regulation/positive
• //rnap/eukaryote/yeast
• iGEM Parts Registry