Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dai Yuanyi
Date created: 2015-09-06 11:00:00
Date modified: 2015-09-07 11:31:12
PcyA+Ho1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1660004_sequence (Version 1) |
Description
In order to work,Cph8 has to form chromophore with PCB biosynthetic genes, where the formation of PCB requires the gene pcyA and hol to function as accounted below. Hol is a sort of Iron red pigment oxidase which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidase from Synechocystis Sauv, functions to turn biliverdin IXalpha (BV) into PCB.We connected gene pcyA（BBa_I15009）and ho1（BBa_I15008）together along with the constitutive promoter(BBa_J23100) to ensure a continuous work of the photoinduction system in our E.coli.
Notes
We used oe-pcr to assemble these parts together seamlessly.Source
pcyA gene and ho1 gene both come from Synechocystis.| Sequence Annotation | Location | Component / Role(s) |
| J23100 B0034 start codon pcyA stop codon pcyA barcode B0034 ho1 start codon ho1 barcode stop codon | 1,35 36,47 48,50 48,750 792,797 798,822 823,834 835,1560 835,837 1561,1585 1558,1560 | promoter feature/promoter ribosome_entry_site feature/rbs start_codon feature/start feature/cds CDS stop_codon feature/stop feature/barcode engineered_tag feature/rbs ribosome_entry_site feature/cds CDS start_codon feature/start engineered_tag feature/barcode stop_codon feature/stop |