BBa_K1660005

BBa_K1660005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1660005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dai Yuanyi
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 12:26:12

RFP controlled by the PompR promoter



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1660005_sequence (Version 1)

Description

PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
We insert PompR(BBa_R0082) sequences into the upstream region of rfp gene, which together is connected to vector PSB1C3 afterwards, and therefore we are able to observe the OmpR promoter efficiency through the control of light.

Notes

We constructed this biobrick through pcr.

Source

This part comes from the stock.

Access Instance Definition
public
public
BBa_R0082
BBa_K516032
OmpR
BBa_K516032
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1660005/1