Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xintian Xu
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-11 06:20:46
frr (from Streptomyces avermitilis, increasing avermectin production)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1668002_sequence (Version 1) |
Description
Frr gene encodes the ribosome recycling factor (RRF), which is involved in the release of ribosomes from the translational post-termination complex for a new round of initiation. In comparison to the wild-type strain, avermectin yield was increased significantly by 3- to 3.7-fold in transformants 31267(pFRR-1139) and 31267(pFRRerm-1139), both of which contained multiple frr copies, compared with that in the control strains. The avermectin productivity of each culture was quantitatively measured by HPLC analysis.Research indicated that frr overexpression promoted cell growth as well as the expression of ave genes (including pathway-specific positive regulatory gene aveR for avermectin biosynthesis and ave structural genes), leading in turn to avermectin overproduction. Different from S-adenosylmethionine synthetase gene (metK), frr gene revealed a ??????copy number effect??????: multiple copies of frr had a greater promoting effect on avermectin production than a single copy in each of the three transformed S. avermitilis strains.
Notes
We used seamless assembly.Source
The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers| Sequence Annotation | Location | Component / Role(s) |
| start coden stop coden cds | 1,3 379,384 1,384 | start_codon feature/start feature/stop stop_codon feature/cds CDS |