BBa_K1669000

BBa_K1669000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1669000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Marina Klemencic

Date created: 2015-09-11 11:00:00

Date modified: 2015-09-13 01:22:29

CtfA + His-tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1669000_sequence (Version 1)

Description

This part includes the CtfA gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli.

This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA.

The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes.

Notes

C-terminal His-tag.

Source

Sequence retrieved from GenBank and codon optimized for expression in E. coli.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1669000/1

Member of these Collections

• iGEM Parts Registry