| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_K1670001_sequence (Version 1)
|
Description
BBa_K1670001 contains the EsaR/I regulated esaRC promoter, an engineered variant of the native esaR-promoter from Erwinia stewartii that contains two EsaR-boxes at the -10 region and between the -10 and -35 region[3] that allow a tighter control of the expression of the genes under the control of PesaRC. The part already contains a codon optimized CFP based on BBa_E0020 with a synthetic ribosome-binding site as a fluorescent reporter. eCFP can be replaced using the NdeI restriction site in the start codon as well as the biobrick suffix.
Notes
two EsaR-boxes for tight controll of eCFP expression
Source
Genome sequence of Erwinia stewartii
