| Types | DnaRegion
|
| Roles | engineered_region
Translational_Unit
|
| Sequences | BBa_K1670005_sequence (Version 1)
|
Description
BBa_K1670001encodes for the regulatory protein EsaR of the EsaR/I quorum sensing system from
Erwinia stewartii . Contrary to most other quorum sensing systems, EsaR works as a repressor rather than an activator. It binds at its corresponding binding box between the -10 and the -35 region of its corresponding promoter and inhibits transcription. Binding of 3OC6-HSL to EsaR induced an allosteric change in the structure that prevents its DNA-binding ability and thus induces expression. If the binding box is positioned shortly upstream of the promotor, EsaR also works as an activator of the respective promoter as long as it can bind to the DNA. We use a D91G variant of the gene that shows higher sensitivity towards 3OC6-HSL [2].
Notes
We use a D91G variant of the gene that shows higher sensitivity towards 3OC6-HSL [2]
Source
Synthesized gBlock according to the sequence information from gene sequencing from Erwinia stewartii GenBank: L32183.1 and [3]
