BBa_K1670008

BBa_K1670008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1670008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Magdalena Hannah Kurteu
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 04:22:52

Tyrosinase



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1670008_sequence (Version 1)

Description




BBa_K1670008 codes for tyrosinase from Marinomonas mediterranea, a gram negative bacterium, which should facilitate expression in E. coli. Tyrosinases are native to several eukaryotic and prokaryotic species including fungi and bacteria. In its endogenous form, the tyrosinase is a part of the melanin biosynthesis pathway, converting L-Tyrosine to L-DOPA and further to dopaquinone. Melanins, dark pigments, protect the bacterial cells and spores against UV radiation, bind cytotoxic heavy metals, confer protection against oxidants, heat, enzymatic hydrolysis, antimicrobial compounds and phagocytosis (1).


Tyrosinase from M. mediterranea requires the chaperone
BBa_K1670009
which is responsible for the delivery of copper to the tyrosinase which enhances production of L-DOPA.(2)




References
1. Claus, Harald ; Decker, Heinz. (2006). Bacterial tyrosinases. Systematic and Applied Microbiology. 29 (1), p3-14.
2. Lopez-Serrano, D., Solano, F. and Sanchez-Amat, A. (2007). Involvement of a novel copper chaperone in tyrosinase activity and melanin synthesis in Marinomonas mediterranea. Microbiology, 153(7), pp.2241-2249.





Notes

Sequence was checked for internal BioBrick restriction sites and codon optimised for E. coli BL21.

Source

Marinomonas mediterranea

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Planning
 
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synbiohub#topLevel
BBa_K1670008/1