Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: JIAQI XU
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 12:46:15
Bxb1 recombinase device
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1675011_sequence (Version 1) |
Description
The Bxb1 integrase is a DNA recombinase, more precisely a member of serine recombinase family. Bxb1 could be divided to two parts, gp35 and gp47. The gp35 part could recognize specific sequences, called attB and attP, and then integrate, invert, or excise dsDNA depending on the orientation of recognition sequences. When it inverts DNA sequence, the attB and attP sequences are changed into attL and attR, as other DNA recombinases do. Another part called Bxb1 gp47 binds to integrase-DNA complex and this complex flips inverts DNA back into original sequence by regenerating attB and attP sequences. So the efficiency of inversion mediated by Bxb1 is half and half.Notes
None.Source
P-atp2 is from the standard part BBa_K1675016. http://parts.igem.org/Part:BBa_K1675016The RBS is from the standard part BBa_B0034. http://parts.igem.org/Part:BBa_B0034
The gene Bxb1 is from the standard part BBa_K907000. http://parts.igem.org/Part:BBa_K907000
| Access | Instance | Definition |
| public public public | BBa_K1675016 BBa_B0034 BBa_K907000 | BBa_K1675016 BBa_B0034 Bxb1_Int |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1675016 BBa_B0034 BBa_K907000 | 1,607 616,627 634,2136 | BBa_K1675016 BBa_B0034 Bxb1_Int |