BBa_K1675026

BBa_K1675026 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1675026

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jing Yang

Date created: 2015-09-14 11:00:00

Date modified: 2015-09-16 09:38:13

P-atp2-B0034-lacz alpha

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1675026_sequence (Version 1)

Description

The natural P-atp2 is an alkali-induced promoter in Corynebacterium glutamicum located in F0F1 ATPase operon. The natural P-atp2 promoter responds to the pH changes from pH 7.0 to pH 9.0, especially at alkaline pH. When the pH value increasing, the expression increased modestly. It is activated by the alternative sigma factor of the RNA polymerase, whose synthesis would be activated when the bacteria are growing at alkaline pH.

The activity of this mutant at different pH is shown in figure 1.

Notes

There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it.

Source

Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides.

The natural P-atp2 is from Corynebacterium glutamicum.

Lacz alpha is from http://parts.igem.org/wiki/index.php?title=Part:BBa_I732006

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1675026/1

Member of these Collections

• iGEM Parts Registry