Types | DnaRegion
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Roles | engineered_region
Reporter
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Sequences | BBa_K1676047_sequence (Version 1)
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Description
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The FOURTH base pair G is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015.
The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.
Notes
pLac was used as it is a constitutive promoter in S. Oneidensis MR1.
Source
The basic parts are from the iGEM registry. Consisting of the pLac promoter BBa_R0011, eGFP BBa_E0040, double terminator BBa_B0015, we ligated the parts( including original wild type RBS) together and perform site targeted mutagenesis on the construct to mutate the WT RBS.