BBa_K1676075

BBa_K1676075 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1676075

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ooi Kean Hean

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 02:51:34

GFP Reporter with Mutant RBS Opt

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1676075_sequence (Version 1)

Description

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The TWELVETH base pair A is substituted to T. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.

Notes

Primers were designed to contain desired mutations for Quickchange PCR

Source

Site targeted mutagenesis was carried out on original RBS , BBa_B0034

Components

Access	Instance	Definition
public	BBa_R0011	lacI+pL
public	BBa_K1676073	BBa_K1676073
public	BBa_E0040	GFP
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011	1,54	lacI+pL
BBa_K1676073	64,75	BBa_K1676073
BBa_E0040	82,801	GFP
BBa_B0015	810,938	BBa_B0015

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1676075/1

Member of these Collections

• iGEM Parts Registry