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BBa_K1676094

BBa_K1676094 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1676094

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ooi Kean Hean

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-25 08:28:19

Mutant 18 of LDH and wildtype LDP

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K1676094_sequence (Version 1)

Description

This construct is an operon for expression of our mutant LDH and wildtype LDP.

Notes

Primers were designed to have the start and stop codon so that they will not be mutated during PCR

Source

Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon.

Components

Access	Instance	Definition
public public public public public public	BBa_R0011 BBa_B0034 BBa_K1676122 BBa_B0034 BBa_K1676076 BBa_B0015	lacI+pL BBa_B0034 BBa_K1676122 BBa_B0034 BBa_K1676076 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011 BBa_B0034 BBa_K1676122 BBa_B0034 BBa_K1676076 BBa_B0015	1,54 64,75 82,1227 1236,1247 1254,2942 2951,3079	lacI+pL BBa_B0034 BBa_K1676122 BBa_B0034 BBa_K1676076 BBa_B0015