Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-25 08:28:09
Mutant 19 of LDH and wildtype LDP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1676095_sequence (Version 1) |
Description
This construct is an operon for expression of our mutant LDH and wildtype LDP.Notes
Primers were designed to have the start and stop codon so that they will not be mutated during PCRSource
Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon.| Access | Instance | Definition |
| public public public public public public | BBa_R0011 BBa_B0034 BBa_K1676123 BBa_B0034 BBa_K1676076 BBa_B0015 | lacI+pL BBa_B0034 BBa_K1676123 BBa_B0034 BBa_K1676076 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_B0034 BBa_K1676123 BBa_B0034 BBa_K1676076 BBa_B0015 | 1,54 64,75 82,1226 1235,1246 1253,2941 2950,3078 | lacI+pL BBa_B0034 BBa_K1676123 BBa_B0034 BBa_K1676076 BBa_B0015 |