BBa_K1676097

BBa_K1676097 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1676097
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 10:41:25

Mutant 1 of Lactate Permease



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1676097_sequence (Version 1)

    Description

    Lactate Permease from Pseudomonas. Function in our project is to transport L-lactate into the Shewanella Oneidensis MR1. This mutant contains the mutation of L105V, F120L, V169A, I190K, D183G

    Notes

    Primers were designed to have the start and stop codon so that they will not be mutated during PCR

    Source

    Random mutagenesis was carried out via PCR to obtain the mutants.

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1676097/1
     

    Attachments

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