Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Prateek Garg
Date created: 2015-09-15 11:00:00
Date modified: 2016-01-25 01:10:59
mCerulean gene cassette with P48 promoter and TldH terminator for lactobacillus
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1678006_sequence (Version 1) |
Description
The gene cassette comprises of T48 lactobacillus promoter, TldH terminator, and mCerulean gene codon optimized for lactobacillus acidophilus (plantarum). It expresses mCerulean specific cyan fluorescence in E. coli as well.Notes
NoneSource
The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006The terminator comes from the following publication: 'Spath et al. Microbial Cell Factories 2012, 11:141'
The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'
The whole cassette was artificially synthesized.
| Sequence Annotation | Location | Component / Role(s) |
| mCereulean BBa_K1678004 RBS terminator Tldh | 76,795 1,60 61,75 796,933 | feature/cds CDS feature/promoter promoter feature/rbs ribosome_entry_site sequence_feature feature/misc |