Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arthur Molcard
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 04:38:06
Vitamin B12 biosensor, composed by a cobalamin riboswitch in front of an mRFP1
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K1678007_sequence (Version 1) |
Description
This part can be used as a Vitamin B12 biosensor, we tested it for the AdoCobalamin form of B12 (AKA Coenzyme B12), but it might be used also to detect CyanoCobalamin, HydroxyCobalamin, MethylCobalamin according to the following publication : "A riboswitch sensor to determine vitamin B12 in fermented foods"Xuan Zhu 2015. We didn't tried this because we were out of time, anyway this would have to be characterized.
Notes
At the end of the riboswitch sequence (just before the fluorescent protein) we might have identified the beginning of a translated sequence coding for the CbiB protein in the natural organism. We had to insert the fluorescent protein in frame with this truncated protein coding sequence and suppress the START codon of the fluorescent protein in order to obtain a fusion fluo protein.Source
We got inspired by the following paper "A riboswitch sensor to determine vitamin B12 in fermented foods" Xuan Zhu 2015.The riboswitch sequence is coming from P. freudenreichii spp. shermanii DSM 20270
| Sequence Annotation | Location | Component / Role(s) |
| Lac Promoter Lac O Riboswitch sequence from Propionibacterium Supposed RBS Supposed CbiB truncated protein mRFP1 without start codon Scar rrnB T1 terminator Predicted riboswitch (aptamer) by Rfam database | 137,168 171,193 200,409 378,385 389,409 413,1087 410,412 1135,1178 249,305 | promoter feature/promoter operator feature/operator sequence_feature feature/dna feature/rbs ribosome_entry_site CDS feature/protein feature/protein CDS feature/scar sequence_feature feature/misc non_covalent_binding_site feature/binding |