Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cun Wei
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 07:45:11
T5 promoter + RBS + 6x His Tag + ftnA + λ
T0 Terminator
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1679028_sequence (Version 1) |
Description
The design is composed of a T5 promoter regulated by double lacI repressor-binding sites, a RBS, a 6x His tag, a ftnA coding sequence and a λ T0 terminator. We created it for expression of ftnA in E.coli for a long time.Notes
We created this device for expression of ftnA in E.coli for along time. T5 promoter is weaker than T7 promoter, so that it can exclude stress responses and adapt to long-time expression. The 6x His tag is design for Ni-chelating affinity chromatography.Source
Synthesized by Genewiz.| Sequence Annotation | Location | Component / Role(s) |
| T5 promoter LacI operator LacI operator RBS 6x His Tag ftnA stop codons λ T0 terminator | 1,48 24,40 56,72 89,94 115,132 139,621 627,637 643,737 | feature/promoter promoter feature/operator operator feature/operator operator feature/rbs ribosome_entry_site tag feature/tag feature/protein CDS feature/stop stop_codon feature/misc sequence_feature |