BBa_K1680014

BBa_K1680014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1680014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katharina Sporbeck

Date created: 2015-09-16 11:00:00

Date modified: 2015-10-27 01:47:18

pRS313 yeast shuttle vector with Biobrick MCS

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K1680014_sequence (Version 1)

Description

A shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.

Notes

contains an ADH Terminator and restrictions sites for the RFC10 standard only in the multiple cloning site, no restrictions sites for RCF10 enzymes in the backbone.

Source

yeast shuttle vector

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
HIS3 promotor	315,502	feature/promoter, promoter
HIS3	503,1162	CDS, feature/cds
f1 ori	1423,1878	sequence_feature, feature/dna
lacZalpha	1661,2077	feature/cds, CDS
T3 promotor	2153,2172	promoter, feature/promoter
lac promotor	2241,2271	feature/promoter, promoter
pUC origin	2595,3183	sequence_feature, feature/dna
ampR	3354,4214	feature/cds, CDS
ampR promotor	4215,4319	feature/promoter, promoter
CEN/ARS yeast centromere	4356,4859	sequence_feature, feature/dna
T7 promotor	2046,2065	promoter, feature/promoter
RFC10 MCS	2083,2125	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1680014/1

Member of these Collections

• //chassis/eukaryote/yeast
• //dna
• //plasmid
• //plasmidbackbone
• //plasmidbackbone/copynumber/low
• iGEM Parts Registry
• igem_plasmid_vectors