BBa_K1680015

BBa_K1680015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1680015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katharina Sporbeck

Date created: 2015-09-16 11:00:00

Date modified: 2015-10-28 06:36:51

pRS315 yeast shuttle vector with Biobrick MCS

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K1680015_sequence (Version 1)

Description

A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bakteria and URA3 gene for yeast selection.

Notes

contains an RFC10 compatible multiple cloning site, no restrictions sites for RFC10 in the Backbone. ADH Terminator after MCS.

Source

yeast shuttle vector

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LEU2	663,1757	feature/cds, CDS
LEU2 promotor	1770,2174	promoter, feature/promoter
f1 origin	2474,2929	feature/dna, sequence_feature
lacZalpha	2712,3128	CDS, feature/cds
T7 promotor	3097,3115	promoter, feature/promoter
RFC10	3134,3176	feature/misc, sequence_feature
lacZalpha	3186,3248	feature/cds, CDS
T3 promotor	3205,3223	feature/promoter, promoter
lac promotor	3292,3322	promoter, feature/promoter
pUC origin	3646,4234	feature/dna, sequence_feature
ampR	4405,5265	feature/cds, CDS
ampR promotor	5266,5370	feature/promoter, promoter
CEN/ARS yeast centromere	5407,5910	feature/dna, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1680015/1

Member of these Collections

• //chassis/eukaryote/yeast
• //dna
• //plasmid
• //plasmidbackbone
• //plasmidbackbone/copynumber/low
• iGEM Parts Registry
• igem_plasmid_vectors