BBa_K1680016

BBa_K1680016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1680016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katharina Sporbeck

Date created: 2015-09-16 11:00:00

Date modified: 2015-10-28 06:37:03

pRS316 yeast shuttle vector with Biobrick MCS

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K1680016_sequence (Version 1)

Description

A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.

Notes

Has an RFC10 compatible MCS, with ADH Terminator. No further RFC10 restrictions sites in the backbone.

Source

yeast shuttle vector

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
URA3 promotor	195,410	promoter, feature/promoter
URA3	411,1214	CDS, feature/cds
f1 origin	1343,1798	feature/dna, sequence_feature
lacZalpha	1581,1997	feature/cds, CDS
T7 promotor	1966,1985	feature/promoter, promoter
RFC10 MCS	2003,2045	sequence_feature, feature/misc
lacZalpha	2055,2117	CDS, feature/cds
T3 promotor	2073,2092	promoter, feature/promoter
lac promotor	2161,2191	promoter, feature/promoter
pUC origin	2515,3103	feature/dna, sequence_feature
ampR	3274,4134	feature/cds, CDS
ampR promotor	4135,4239	feature/promoter, promoter
CEN/ARS yeast centromere	4276,4779	feature/dna, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1680016/1

Member of these Collections

• //chassis/eukaryote/yeast
• //dna
• //plasmid
• //plasmidbackbone
• //plasmidbackbone/copynumber/low
• iGEM Parts Registry
• igem_plasmid_vectors