Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zilu Peter Tang
Date created: 2015-08-25 11:00:00
Date modified: 2015-09-06 05:02:57
conjugation(RP4) plasmid with MHT (Methyl halide transferase) and GFP
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1682010_sequence (Version 1) |
Description
This plasmid contains three main components: the RP4, GFP, and MHT. The RP4 gene, when transformed specifically into S17 E. coli strain, facilitates the conjugation of E.coli with most other bacteria. The plasmid also contains MHT, short for methyl halide transferase, which if provided with either Br, Cl, or I, produces its corresponding methyl halide gas. This part also contains a GFP, specifically BBa_E0040. This plasmid can be used to faciliate transformation of bacteria that are not easily transformable through heat shock or electroporation. In addition, the plasmid also have two reporter genes, which produce the GFP and the methyl halide gas.Notes
N/ASource
MHT sequence comes from organism Batis maritima. GFP comes from the biobrick BBa_E0040. RP4 comes from gBlock pRZ101| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon RP4 gene GFP BBa_0040 Methyl Halide Transferase | 1,3 1,688 711,1430 1783,2535 | start_codon feature/start feature/dna sequence_feature engineered_region feature/BioBrick feature/protein CDS |