BBa_K1683003

BBa_K1683003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1683003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew W Mortensen
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-16 11:48:31

E. coli tolC signaling sequence + Proteus mirabilis tolC



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1683003_sequence (Version 1)

Description

The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Proteus mirabilis. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump.

Notes

The main consideration when designing the signaling sequence and Proteus mirabilis TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.

Source

The tolC signaling sequence and Proteus mirabilis tolC sequence were synthesized from DNA sequences found in genomic databases

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1683003/1