| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1683003_sequence (Version 1)
|
Description
The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Proteus mirabilis. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump.
Notes
The main consideration when designing the signaling sequence and Proteus mirabilis TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.
Source
The tolC signaling sequence and Proteus mirabilis tolC sequence were synthesized from DNA sequences found in genomic databases
